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目的:应用流式细胞检测技术检测肺炎支原体患者外周血T淋巴细胞亚群的变化,对结果进行初步的探讨,初步分析肺炎支原体肺炎患者T淋巴细胞亚群变化的意义。方法:选取2012年6月至2013年6月我院儿科收治的肺炎支原体肺炎患儿45例作为本次研究的对象,37例健康小儿作为其对照组研究对象。所有研究对象均抽取静脉血3ml,处理后采用手工试剂盒法检测CD3、CD4、CD8细胞相对数量,借此观察外周血T淋巴细胞变化情况。结果:肺炎支原体患者外周血CD8表达显著高于健康患儿,而CD3、CD4及两者的比例显著低于正常健康患儿。所有比较结果差异显著,均有统计学意义,P值均<0.05。结论:肺炎支原体患儿外周血T淋巴细胞亚群失衡,可能是疾病发生发展的原因。
OBJECTIVE: To detect the changes of T lymphocyte subsets in peripheral blood of patients with Mycoplasma pneumoniae by flow cytometry, and to discuss the preliminary results of this study. The significance of the changes of T lymphocyte subsets in patients with Mycoplasma pneumoniae pneumonia was preliminarily analyzed. Methods: Forty-five children with mycoplasma pneumoniae pneumonia admitted in our hospital from June 2012 to June 2013 were selected as the object of this study and 37 healthy children were selected as the control group. All subjects were drawn venous blood 3ml, after treatment using manual kit method to detect the relative number of CD3, CD4, CD8 cells, to observe the changes of peripheral blood T lymphocytes. Results: The expression of CD8 in peripheral blood of patients with Mycoplasma pneumoniae was significantly higher than that of healthy children, while the proportions of CD3, CD4 and both were significantly lower than those in healthy children. All the results were significant differences, both statistically significant, P <0.05. Conclusion: The imbalance of T lymphocyte subsets in children with Mycoplasma pneumoniae may be the reason of disease development.