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目的:应用正交试验法研究活血理气颗粒提取纯化工艺,并用小鼠镇痛抗炎实验探究提取方案优化后所提取浸膏的药效。方法:采用正交设计L9(34)方法,每次试验按处方量称取药材(225g)作为投料量,以干浸膏得率为考察指标,优选最佳的提取纯化工艺参数。结果:最佳提取纯化工艺为A3B1C3,即加水10倍量,每次1小时,煎煮3次,镇痛抗炎疗效显著。结论:通过正交试验设计筛选出的提取纯化方法较好,该法所得浸膏小鼠镇痛抗炎实验的药效观察疗效显著,工艺合理可行。
OBJECTIVE: To study the extraction and purification technology of Huoxue Liqi granule by orthogonal test, and to study the pharmacodynamics of extracted extract by optimizing the extraction scheme with mouse analgesic and anti-inflammatory test. Methods: The orthogonal design L9 (34) method was used. The dosage of herbs (225g) was weighed as the prescription amount in each trial. The yield of dry extract was taken as the index to evaluate the optimal extraction and purification process parameters. Results: The best extraction and purification process for the A3B1C3, that is, 10 times the amount of water, 1 hour each, boiling 3 times, analgesic and anti-inflammatory effect was significant. Conclusion: The method of extraction and purification screened by orthogonal design is better. The efficacy of analgesic and anti-inflammatory experiment of the extract mice obtained by this method is significant and the process is reasonable and feasible.