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目前,基因转移的方法已发展到很多作物上,但大多都不是很有效的,并且对于育种家来说又太复杂。本项实验的目的是研究能否通过将外源DNA注入幼荚的方法来转化大豆。实验中,利用一个玉米自交系和一个栽培大豆品种作为供体,另一个栽培大豆品种作为受体。两个组合的突变率分别为10.00%和5.32%。后代在很多农艺性状方面产生了可遗传的变异,例如:熟期、种子蛋白质含量、株高、结荚习性、茎粗、倒伏性、每株分枝数、每株荚数、每株粒数和百粒重。外源DNA可以通过维管系统或胞间连丝系统进入分裂时期的细胞,或通过核膜通道进入细胞核,然后聚集到基因并在受体上得到表达。该项技术简单、有效、育种家容易掌握。利用该技术可创造新的种质,实现不同作物种间、属间、种间基因交换。
At present, methods of gene transfer have been developed on many crops, but most are not very effective and are too complicated for breeders. The purpose of this experiment was to investigate whether it is possible to transform soybeans by injecting foreign DNA into young pods. In the experiment, one maize inbred line and one cultivated soybean variety were used as donors, and another cultivated soybean variety was used as the recipient. The mutation rates of the two combinations were 10.00% and 5.32% respectively. Offspring have inherited variations in many agronomic traits, such as: maturity, seed protein content, plant height, pod habit, stem diameter, lodging, number of branches per plant, number of pods per plant, number of grains per plant And 100 weight. Exogenous DNA can enter the dividing cell through the vascular system or the intercellular connection system, or enter the nucleus through the nuclear membrane channel, then gather into the gene and express on the receptor. The technique is simple and effective and breeders are easy to master. The use of this technology can create new germplasm to achieve intercropping, interspecific and interspecific genetic exchange among different crops.