论文部分内容阅读
目的:研究忍冬叶总黄酮(TFLLJ)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分为正常组、模型组、TFLLJ高、中、低剂量组(400,200,100 mg.kg-1)和联苯双酯组(150 mg.kg-1),除正常组和模型组外,其余各组小鼠每日ig给药1次,共10 d。末次给药1 h后,除正常组外其余各组ip 0.1%CCl4花生油溶液(0.01 mL.g-1)1次,造成急性肝损伤。测定血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)含量,计算肝脏指数(LI),测定肝组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,观察肝组织病理改变。结果:TFLLJ(400,200,100 mg.kg-1)剂量组可降低CCl4致小鼠肝损伤血清中的ALT和AST(P<0.01或P<0.05)。可降低小鼠肝组织中MDA和SOD活性(P<0.01或P<0.05)。TFLLJ各剂量组能明显改善肝组织的病理变化。结论:忍冬叶总黄酮对CCl4致小鼠急性肝损伤具有一定的保护作用,可能与抗脂质氧化有关。
Objective: To study the protective effect of total flavonoids of honeysuckle leaves (TFLLJ) on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: Sixty Kunming mice were randomly divided into normal group, model group, TFLLJ high, medium and low dose groups (400,200,100 mg.kg-1) and bifendate group (150 mg.kg-1) Normal group and model group, the other groups of mice daily ig administration 1, a total of 10 d. At 1 h after the last administration, the rats in each group were treated with ip 0.1% CCl 4 peanut oil solution (0.01 mL.g-1) for 1 time, causing acute liver injury. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured. Liver index (LI) was calculated. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue were measured. Results: TFLLJ (400, 200 and 100 mg.kg-1) dose groups could reduce ALT and AST levels in serum of CCl4-induced liver injury (P <0.01 or P <0.05). Can reduce the activity of MDA and SOD in the liver of mice (P <0.01 or P <0.05). TFLLJ each dose group can significantly improve the pathological changes of liver tissue. CONCLUSION: The total flavonoids of Lonicera japonica leaves have a protective effect on acute liver injury induced by CCl4 in mice, which may be related to anti-lipid oxidation.