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目的:通过比较不同年份红树林修复区的根际微生物结构,建立微生物区系与修复质量的相关性,为红树林修复提供理论借鉴。方法:采集4年、8年、10年红树林修复区以及原生红树林的根际微生物,利用末端限制性片段长度多态性技术(Terminal Restriction Fragment Length Polymorphism,T-RFLP)、聚类分析(Cluster Analysis,CA)、主成分分析法(Principal Component Analysis,PCA)、α多样性分析法、文氏图、稀释性曲线等分析方法,研究不同样本间根际微生物的群落组成并进行差异性对比。结果:通过分析不同修复年限间根际微生物的短片段重复序列(Short Tandem Repeat,STR)测序结果,发现三个不同修复时间的红树林修复区和原生红树林根际微生物间均存在差异,10年修复区更加接近原生红树林,4年和8年修复区享有更加相似的微生物群落,且同原生红树林间存在较大差异。结论:修复区红树林的根际微生物群落结构会逐步向着原生红树林根际微生物群落结构演替,但这一过程并非简单的加和式线性变化,而是存在复杂性和年份突变性的可能。
OBJECTIVE: To establish the correlation between microbial flora and restoration quality by comparing rhizosphere microbial structure in mangrove restoration area of different years and provide theoretical reference for mangrove restoration. Methods: Rhizosphere microbes were collected from mangrove restoration areas and native mangrove forests at 4, 8, and 10 years. T-RFLP, T-RFLP and cluster analysis Cluster Analysis, CA), Principal Component Analysis (PCA), α diversity analysis, Venturi plot and dilution curve were used to study the composition of rhizosphere microbes in different samples and their differences were compared . Results: By analyzing the short tandem repeat (STR) sequence of rhizosphere microbes in different years of remediation, it was found that there were differences in rhizosphere microorganisms of mangrove restoration area and virgin mangrove at three different restoration times, 10 The annual restoration areas are closer to the native mangroves, and the four and eight years restoration areas enjoy more similar microbial communities, which are quite different from the native mangroves. Conclusion: The rhizosphere microbial community structure of the mangroves in the rehabilitated zone will gradually follow the succession of microbial community structure in the rhizosphere of native mangrove forests. However, this process is not a simple additive linear change, but may have the complexity and year mutation possibility .