采用Mueller氏检测ANAE的组织化学方法,在615小鼠的淋巴结、脾、胸腺中发现ANAE阳性细胞主要分布于T细胞区,而ANAE阴性细胞主要分布于B细胞区,因而可以认为ANAE阳性时出现的红棕色粗结状颗粒为T淋巴细胞的标记。将此法应用于我国自行培育的615近交系小鼠24例的血片检测中,发现ANAE阳性“T”细胞占全部淋巴细胞的55± By means of histochemical analysis of the ANAE by the Mueller’s method, ANAE-positive cells were mainly distributed in the T-cell region in the lymph nodes, spleen, and thymus of 615 mice, while ANAE-negative cells were mainly distributed in the B-cell region, so that it can be considered that the ANAE was positive. The red-brown rough knotted granules are markers of T lymphocytes. This method was applied to the blood test of 24 of 615 inbred mice cultivated in China. It was found that ANAE-positive “T” cells accounted for 55± of all lymphocytes.