目的 :比较分析 3种测定蛇胆汁中主要成分牛磺胆酸含量的方法 ,制定科学合理统一的质量标准。方法 :采用薄层层析 磷钼酸比色法、薄层扫描法及高效液相色谱法对蛇胆汁中主要成分牛磺胆酸进行测定 ,并将结果进行比较分析。结果 :薄层层析 磷钼酸比色法、薄层扫描法及高效液相色谱法的含量测定结果有一定差异 ,平均回收率分别为 97.4 8% ,98.51 %和 1 0 0 .3 4 % ,RSD分别为 4 .6% ,4 .3 %和 1 .5%。结论 :高效液相色谱法灵敏、准确 ,可作为测定蛇胆汁中主成分牛磺胆酸含量的法定分析方法 ,建议制定统一的质量标准。 OBJECTIVE: To compare and analyze three methods for determination of taurocholic acid, the main component of snake bile, and to establish a scientific and reasonable quality standard. Methods: The content of taurocholic acid in snake bile was determined by TLC-TLC, TLC and HPLC. The results were compared and analyzed. Results: The results of TLC, TLC and HPLC showed that the average recoveries were 97.48%, 98.51% and 1.04%, respectively , RSD were 4.6%, 4.3% and 1.5% respectively. Conclusion: HPLC is sensitive and accurate and can be used as a statutory method for the determination of taurocholic acid in the main components of snake bile. It is suggested that a uniform quality standard should be established.