目的　构建弓形虫速殖子期表达的基因的完整长度cDNA文库。方法　用CF - 11纤维素柱快速提纯速殖子 ,以盐酸异硫氰酸胍 (AGPC)一步法抽提总RNA ,oligo -dT纤维素分离mRNA后 ,用ClonTech公司的SmartTMPCRcDNA文库构建试剂盒 ,构建了弓形虫昆山分离株的表达型文库。结果　获得 5× 10 6个独立克隆 ,重组率为 99% ,插入片段的平均长度为 1kb。结论　本文库可望用于弓形虫疫苗研究的候选基因的筛选 Objective To construct a full length cDNA library of tachyzoites expressed in tachyzoites. Methods Totally purified triton with CF - 11 cellulose column, one step extraction of total RNA with guanidinium isothiocyanate (AGPC), oligo - dT cellulose mRNA isolation, using ClonTech SmartTMPCR cDNA library construction kit, The Toxoplasma gondii Kunshan isolate expression library was constructed. As a result, 5 × 10 6 independent clones were obtained, the recombination rate was 99%, and the average length of the insert was 1 kb. Conclusion This library is expected to be used in the screening of candidate genes for Toxoplasma gondii vaccine research