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目的:观察加味鳖甲煎丸对四氯化碳(CCl4)所致肝纤维化大鼠肝组织的保护作用。方法:由皮下注射40%CCl4溶液首次5 mL/kg,以后每周2次,每次3 mL/kg,连续注射6周,在病理检查证明造模大鼠肝组织出现肝纤维化后,分组给药,连续给药8周后处死大鼠,病理切片,HE和Masson染色,观察加味鳖甲煎丸对CCl4所致肝纤维化大鼠肝组织病变程度的影响,并对脂质空泡和胶原含量进行定量分析。结果:加味鳖甲煎丸大、中剂量组大鼠肝组织炎性细胞浸润程度、脂质空泡和胶原含量、肝假小叶形成的数量均较模型组大鼠明显减少,肝纤维化病变程度明显减轻。结论:加味鳖甲煎丸对CCl4所致肝纤维化大鼠肝组织有较好的保护作用,可明显减轻其病变程度。
OBJECTIVE: To observe the protective effect of Jiawei Biejiajian Pill on hepatic tissue in hepatic fibrosis rats induced by carbon tetrachloride (CCl4). Methods: Subcutaneous injection of 40% CCl4 solution for the first time 5 mL/kg, then 2 times per week, each time 3 mL/kg, continuous injection for 6 weeks, after pathological examination to prove liver tissue hepatic fibrosis in model rats, grouping After administration for 8 weeks, the rats were sacrificed. Pathological sections were stained with HE and Masson to observe the effect of Jiawei Biejiajian Pills on the degree of hepatic tissue damage in rats with hepatic fibrosis induced by CCl4, and the lipid vacuoles and Collagen content was quantified. RESULTS: Compared with the model group, the amount of inflammatory cell infiltration, lipid vacuoles and collagen content, and the number of hepatic lobular lobule formed in the rats in the medium-dose and medium-dose group of Jiawei Biejia Jianwan were significantly reduced, and the degree of hepatic fibrosis was significant. Reduce. Conclusion: Jiawei Biejiajian Pill has a good protective effect on liver tissue of rats with liver fibrosis induced by CCl4, and can significantly reduce the degree of its lesions.