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分析了田间栽培条件下2年生转UGPase基因喜树与对照株的木材化学成分与生长速率。结果表明,转UGPase基因喜树综纤维素含量达到78.87%,比对照株相比提高了2.23%;纤维素含量为36.34%,与对照株相比没有明显提高;木质素含量为15.05%,较对照株降低了1.75%;两者的灰分含量均较低且无显著差异;冷、热水抽提物含量为7.62%与10.17%,分别提高了2.04%与2.13%;1%NaOH抽提物含量为27.13%,提高了1.27%。因此,就综纤维素、木质素、灰分含量而言,转UGPase基因喜树为优质纸浆材,水抽提物和1%NaOH抽提物的含量略高,在纸浆生产中需加以重视。本文还对转UGPase基因喜树与对照株的株高、基径、生物量进行了动态监测,结果表明,从5月25日到11月10日的生长季中,其株高平均增加121 cm,对照株平均仅67.8 cm,株高生长速率提高了78.47%;基径平均增加1.792 cm,对照株平均仅0.532 8 cm,提高了236.37%;地上部分生物量的积累与对照相比提高了322.61%,即转入UGPase基因使喜树生长速率显著提高。因此,虽然转UGPase基因喜树的综纤维素和纤维素含量没有明显提高,但其生长速率快,生物量增长显著,间接提高了纤维素与喜树碱的产量。因此,转基因喜树较普通喜树更符合纸浆材速生、纤维素含量高和产量高、木质素含量低的基本要求,可在生产中进一步推广。
The wood chemical composition and growth rate of two-year-old UCPase gene camptotheca acuminata and control plants under field cultivation were analyzed. The results showed that the content of cotyledons of UGPase gene was 78.87%, which was 2.23% higher than that of the control strain. The content of cellulose was 36.34%, which was not significantly higher than that of the control strain. The content of lignin was 15.05% The control strain was reduced by 1.75%; the ash content of both was lower and no significant difference; cold and hot water extract content of 7.62% and 10.17%, respectively, increased 2.04% and 2.13%; 1% NaOH extract The content of 27.13%, an increase of 1.27%. Therefore, in terms of holocellulose, lignin, and ash content, the Camptotheca acuminata-UGPase gene was a good pulp material with a slightly higher content of water extract and 1% NaOH extract, which needs attention in pulp production. In this paper, we also dynamically monitored the plant height, basic diameter and biomass of camptotheca acuminate (UGPase) gene and control plants. The results showed that the plant height increased by 121 cm on average during the growing season from May 25 to November 10 The average growth rate of plant height was increased by 78.47%, the average diameter of base increased by 1.792 cm, the average value of control plant was only 0.532 8 cm, increased by 236.37%; the accumulation of aboveground biomass increased by 322.61 compared with the control %, That is transferred to the UGPase gene Camptotheca acuminata growth rate significantly increased. Therefore, although the content of holocellulose and cellulose in Camptotheca acuminata-UGPase transgenic tree did not increase significantly, its growth rate was rapid and its biomass increased significantly, which indirectly increased the yield of cellulose and camptothecin. Therefore, transgenic Camptotheca acuminatum is more in line with the basic requirements of the rapid growth of pulpwood, high cellulose content and high yield, low lignin content, can be further promoted in production.