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用脊髓灰质炎病毒(Poliovirus)减毒活疫苗株(中Ⅲ-2)感染人二倍体胚肺成纤维细胞株(KMB17)后,细胞形态变化分为两个阶段:第一阶段是致CPE过程,导致形态学上特有的细胞圆缩、体积缩小等CPE特征,经光学显微镜、荧光显微镜、细胞流式仪、电子显微镜、DNA凝胶电泳分析,证明该疫苗株诱导的细胞病理改变具典型的凋亡特征;细胞圆缩、细胞核浓缩破裂、核染色质凝缩后分布于核膜边缘、绝大部分细胞出现在凋亡区域、DNA断裂。表明脊髓灰质炎病毒诱导的CPE本质是一个诱导细胞凋亡过程;第二阶段是Poliovirus诱导细胞坏死。进一步提示有可能通过抑制细胞凋亡提高脊髓灰质炎减毒活疫苗的产量。
After inoculation of human diploid embryo lung fibroblast line (KMB17) with a live attenuated poliovirus vaccine strain (Medium III-2), the cell morphological changes were divided into two stages: the first stage was CPE Process, resulting in morphology-specific CPE features such as rounding and shrinking of cells. The results of optical microscope, fluorescence microscope, flow cytometry, electron microscopy and DNA gel electrophoresis showed that the cytopathic changes induced by this vaccine strain were typical The apoptotic cells were round and the nucleus condensed and ruptured. The nuclear chromatin condensed and distributed on the edge of the nuclear membrane, most of the cells appeared in the apoptotic area and the DNA was broken. It shows that the nature of CPE induced by poliovirus is a process of inducing apoptosis. In the second stage, Poliovirus induces cell necrosis. It is further suggested that it is possible to increase the yield of live attenuated polio vaccine by inhibiting apoptosis.