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高粱是世界上第五大主要粮食作物,也是非洲国家的主要粮食来源之一,Striga asiatica是一种寄生于高粱等主要农作物的野生草之一。选用两个对寄生草抗性表现差异的 高粱品系“山桂红”和“SRN39”作亲本,构建了一个重组近交系群体(Recombinant inbred, RI),并随机筛选出94个系用于构建分子连锁图谱和基因定位。在应用的286个多态性标记 中,有251个标记分别标定在10条不同的连锁群上,标记间的平均图距为7.1cm,总图谱覆盖 了高粱基因组的1779cm,是目前国际上几个比较完整的高粱分子连锁图谱之一。群体的共 分离分析表明,与寄生草抗性有关的萌发诱导物基因(Germinination stimulant gene-GermStim) 位于高粱的遗传连锁群J上,相距较近的分子标记为13cm。进一步的精细定位分析,发现有 两个分子标记分别位于基因的两侧,距离为1.6和2.1个cm。
Sorghum is the fifth-largest staple food crop in the world and one of the staple food sources in African countries. Striga asiatica is one of the wild grass parasitized on major crops such as sorghum. Two recombinant sorghum strains “Shan Gui Hong” and “SRN39” were selected as parents to construct a recombinant inbred population (RI), and 94 strains were screened out randomly to construct Molecular linkage map and gene mapping. Of the 286 polymorphic markers used, 251 markers were mapped on 10 different linkage groups respectively. The average distance between markers was 7.1 cm. The total population covered 1779 cm of sorghum genome. A few more complete molecular linkage map of sorghum. Co-segregation analysis of the populations showed that Germinative stimulant gene-GermStim was located on the genetic linkage group J of sorghum, and the closest molecular marker was 13 cm. Further fine positioning analysis revealed two molecular markers located on each side of the gene at distances of 1.6 and 2.1 cm.