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比较TRAPPCRELISA及TRAPPCRPAGE检测鼻咽癌端粒酶的活性。方法 :分别采用TRAPPCRELISA及TRAPPCRPAGE检测了 38例鼻咽癌 (NPC)、15例癌旁组织、19例慢性鼻咽炎和鼻咽癌HNE1细胞株、其它 2种癌细胞株及 3种正常细胞的端粒酶表达。并探讨了在不同数量的HNE1细胞中端粒酶表达的灵敏度及HNE1细胞株、5例NPC活检组织经热灭活后端粒酶检测的特异性。结果 :两种方法在NPC、癌旁、慢性鼻咽炎及HNE1细胞株端粒酶表达的阳性率非常接近 ,分别为 84.2 9%和 85 .2 9%、73.3%和6 9.2 %、5 .3%和 12 .1%及 95 .5 %和 91.2 %。ELISA间接定量法能很好地反映不同程度的端粒酶活性状态。在 10 2 HNE1细胞中仍能检测到端粒酶活性 ,而热灭活后测不出其活性。结论 :两种方法均能很好地显示鼻咽部不同组织及细胞中的端粒酶活性状态 ,有较高的灵敏度和特异性。
The TRAPPCRELISA and TRAPPCRPAGE were used to detect telomerase activity in nasopharyngeal carcinoma. Methods: TRAPPCRELISA and TRAPPCRPAGE were used to detect 38 cases of nasopharyngeal carcinoma (NPC), 15 cases of paracancerous tissues, 19 cases of chronic nasopharyngitis and nasopharyngeal carcinoma HNE1 cell lines, the other two kinds of cancer cell lines and three normal cells Granzyme expression. The sensitivity of telomerase expression in different numbers of HNE1 cells and the specificity of telomerase detection in heat-inactivated HNE1 cell lines and 5 NPC biopsies were explored. RESULTS: The positive rates of telomerase expression in NPC, paracancerous, chronic nasopharyngitis and HNE1 cell lines were very close, 84.2 9% and 85.29%, 73.3% and 62.2%, respectively % And 12.1% and 95.5% and 91.2% respectively. ELISA indirect quantification method can well reflect the different degree of telomerase activity status. Telomerase activity was still detectable in 102 HNE1 cells, but no activity was detected after heat inactivation. Conclusion: Both of the two methods can display the telomerase activity in different tissues and cells of nasopharynx well, with high sensitivity and specificity.