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Artificial molecular chaperone(AMC)and ion exchange chromatography(IEC)were integrated,thus a new refolding method, artificial molecular chaperone-ion exchange chromatography(AMC-IEC)was developed.Compared with AMC and IEC,the activity recovery of lysozyme obtained by AMC-IEC was much higher in the investigated range of initial protein concentrations, and the results show that AMC-IEC is very efficient for protein refolding at high concentrations.When the initial concentration of lysozyme is 180 mg/mL,its activity recovery obtained by AMC-IEC is still as high as 76.6%,while the activity recoveries obtained by AMC and IEC are 45.6% and 42.4%,respectively.
Artificial molecular chaperone (AMC) and ion exchange chromatography (IEC) were integrated, thus a new refolding method, artificial molecular chaperone-ion exchange chromatography (AMC-IEC) was developed. Compared with AMC and IEC, the activity recovery of lysozyme obtained by AMC-IEC was much higher in the investigated range of initial protein concentrations, and the results show that AMC-IEC is very efficient for protein refolding at high concentrations. When the initial concentration of lysozyme is 180 mg / mL, its activity recovery obtained by AMC-IEC is still as high as 76.6% while the activity recoveries obtained by AMC and IEC are 45.6% and 42.4% respectively.