对于完整或较完整的人体骨骼，根据骨的形态特点或采用仪器测量、X线检查等进行骨性别判定，其准确性较高。而在碎尸、白骨化、生物考古、灾难性事故等情况残存的遗骸就难以用形态学方法确定性别。本文采用PCR方法，特异性扩增骨组织X－Y染色体DNA同源性基因序列，鉴定骨组织的 For complete or more complete human bones, according to the morphological characteristics of the bone or the use of instrument measurement, X-ray examination for bone sex determination, its accuracy is higher. However, it is difficult to determine the gender by the morphological method in remnants of the remains such as corpse, white bone, biological archeology and catastrophic accident. In this paper, PCR method, specific amplification of bone X-Y chromosome DNA homology gene sequence, identification of bone tissue