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目的探讨慢病毒介导碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)基因修饰的骨髓基质细胞(bone marrow stromal cells,BMSCs)对脑梗死后动脉生成的影响。方法利用慢病毒载体介导b FGF基因修饰BMSCs,获得稳定转染b FGF的BMSCs。脑梗死后1 d立体定向移植至梗死灶周围。术后14 d后采用免疫组织化学染色法标记梗死灶周围微小动脉,并采用MCID计算机成像分析系统测量微小动脉的密度及直径。结果与对照组比较,BMSCs组及b FGF-BMSCs组微小动脉密度及直径明显增加(P<0.05),且b FGF-BMSCs组微小动脉密度及直径显著高于BMSCs组(P<0.05)。结论 b FGF修饰的BMSCs可有效促进脑梗死后动脉生成。
Objective To investigate the effect of leukemia-induced bone marrow stromal cells (BMSCs) modified by basic fibroblast growth factor (bFGF) gene on the formation of artery after cerebral infarction. Methods BMSCs were transfected with bFGF gene modified lentiviral vector to obtain BMSCs stably transfected with bFGF. Stent stereotactic grafting around the infarct 1 d after cerebral infarction. At 14 days after operation, the small arteries around the infarct were labeled by immunohistochemical staining. The density and diameter of the small arteries were measured by MCID imaging system. Results Compared with the control group, the density and diameter of arterioles in BMSCs group and b FGF-BMSCs group were significantly increased (P <0.05). The density and diameter of arterioles in bFGF-BMSCs group were significantly higher than those in BMSCs group (P <0.05). Conclusion b FGF-modified BMSCs can effectively promote the formation of artery after cerebral infarction.