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为了对囊尾蚴病的免疫诊断提供有价值的单克隆抗体(McAh),我们应用杂交瘤技术,通过对骨髓瘤细胞NS-1与用猪囊尾蚴囊液抗原免疫的BALB/C小鼠脾细胞融合试验及反复筛选和多次克隆化,建立了3株分泌抗猪囊尾蚴抗原的McAb杂交瘤细胞株1D_4、4E_(11)和4E_(12)。其分泌的抗体具有很强的种的特异性,与细粒棘球蚴和肥颈绦虫囊尾蚴抗原均不发生交叉反应。免疫球蛋白亚类鉴定表明3株均属IgGI。经蛋白质转移电泳试验(Western blot)确定,与所获McAb发生特异反应的抗原是猪囊尾蚴头节囊壁(SCW)抗原分子量为25kDa和17kDa的蛋白组分。
In order to provide a valuable monoclonal antibody (McAh) for immunodiagnosis of cysticercosis, we used hybridoma technology to detect myeloma cells by immunizing myeloma cells NS-1 with BALB / c mouse splenocytes immunized with Cysticercus cellulosae antigen Three McAbs hybridoma cell lines, 1D_4, 4E_ (11) and 4E_ (12) secreting anti-Cysticercus cellulosae antigen, were established by fusion test, repeated screening and multiple cloning. The secreted antibodies have a strong species-specific, and Echinococcus granulosus and Feiyitai cecrops antigen do not cross-react. Immunoglobulin subclass identification showed that all three strains were IgGI. Western blot analysis showed that the McAbs that reacted specifically with McAbs were the protein components of 25 kDa and 17 kDa molecular weight of cysticercus cellulosae.