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T细胞在机体应对外来抗原的免疫应答过程中处于其中心环节。木犀草素(luteolin)有抗炎、抗氧化、抗肿瘤、抗变应性、抗病毒和细胞保护作用。研究中,以佛波醇酯(PMA)+离子霉(Ion)素刺激小鼠淋巴细胞来源的T细胞,以不同终浓度的木犀草素与T细胞共培养,MTT法检测发现,PMA+Ion刺激48 h后,小鼠T淋巴细胞增殖显著高于对照组;木犀草素(10、20、30μmol/L)可显著降低PMA+Lon引起的细胞增殖;Western blot检测发现,PMA刺激T细胞30 min时能显著促进胞浆中的p65进入核,木犀草素(10、20、30μmol/L)能抑制PMA刺激的T细胞p65入核,以30μmol/L的木犀草素抑制作用最强。研究表明,木犀草素能通过抑制NF-κB p65的入核,抑制小鼠T细胞的增殖和活化,调节机体免疫功能。
T cells are at the center of their immune responses to foreign antigens. Luteolin has anti-inflammatory, anti-oxidant, anti-tumor, anti-allergic, anti-viral and cytoprotective effects. In the study, mouse lymphocyte-derived T cells were stimulated with phorbol ester (PMA) + ionon (Ion), co-cultured with luteolin at different final concentrations and T cells, and detected by MTT assay, PMA+Ion After stimulation for 48 h, the proliferation of mouse T lymphocytes was significantly higher than that of the control group; luteolin (10, 20, 30 μmol/L) could significantly reduce the cell proliferation induced by PMA+Lon; Western blot analysis found that PMA stimulated T cells 30 At the time of min, it can significantly promote the nuclear import of p65 into the nucleus. Luteolin (10, 20, 30 μmol/L) can inhibit the P65 nuclear importation of PMA-stimulated T cells, and the inhibitory effect of luteolin at 30 μmol/L is the strongest. Studies have shown that luteolin can inhibit the entry of NF-κB p65 into the nucleus, inhibit the proliferation and activation of mouse T cells, and regulate the immune function of the body.