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目的探讨IBP对SACC细胞抗紫杉醇凋亡能力的影响及其机制。方法将ACC2转染IBP组和空白对照组各自根据不同紫杉醇浓度分成5组,紫杉醇作用72 h后,MTT法检测在紫杉醇作用下IBP对SACC细胞增殖的影响;通过微管蛋白Tubulin免疫荧光染色,观察紫杉醇作用前后ACC2细胞微管的变化及IBP与微管的关系。结果 IBP使ACC2细胞对紫杉醇产生一定程度的耐药,在5μg/ml的紫杉醇浓度下最为明显;紫杉醇开始作用后,ACC2-C1细胞的微管点状聚集成团,而ACC2-C1/IBP细胞的微管则出现明显的紊乱、断裂,IBP能促进微管的解聚;IBP所发的绿色荧光与微管的红色荧光糅合在一起呈黄色,IBP与微管存在一定程度的共定位。结论 IBP促进SACC细胞对紫杉醇耐药。
Objective To investigate the effect of IBP on the anti-paclitaxel apoptosis in SACC cells and its mechanism. Methods ACC2 transfected IBP group and blank control group were divided into 5 groups according to the concentration of paclitaxel. After paclitaxel treatment for 72 hours, MTT assay was used to detect the effect of paclitaxel on the proliferation of SACC cells. Tubulin immunofluorescence staining, The changes of microtubules in ACC2 cells and the relationship between IBP and microtubules before and after taxol treatment were observed. Results IBP induced a certain degree of resistance to paclitaxel in ACC2 cells, most notably at the concentration of 5μg / ml paclitaxel. After paclitaxel began to act, microtubules of ACC2-C1 cells aggregated into clusters, while ACC2-C1 / IBP cells Of the microtubules showed obvious disturbance and breakage. IBP could promote the depolymerization of microtubules. The green fluorescence of IBP and the red fluorescence of microtubules were yellow together, and there was a certain degree of colocalization between IBP and microtubules. Conclusions IBP can promote paclitaxel resistance in SACC cells.