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目的:探讨DNA去甲基化试剂5-氮杂-2’-脱氧胞苷(5-Aza-CdR)对宫颈癌Hela细胞株增殖及抑癌基因CDH1和HPV18E6 mRNA的表达水平的影响。方法:不同浓度5-Aza-CdR干预Hela细胞株24h以后,四唑氮蓝法(MTT)检测其对Hela细胞增殖的影响,半定量RT-PCR检测CDH1及HPV18E6 mRNA表达的改变。结果:与对照组(DMSO)相比,5-Aza-CdR能显著抑制Hela细胞增值,且呈量-效性。5-Aza-CdR能显著抑制Hela细胞CDH1 mRNA的表达水平(P<0.05),而对HPV18E6 mRNA表达水平无明显影响(P>0.05)。结论:5-Aza-CdR能抑制Hela细胞增殖,其作用可能是通过上调宫颈癌抑癌基因CDH1的表达实现,有望为宫颈癌的治疗提供新的治疗思路。
AIM: To investigate the effects of 5-Aza-2’-deoxycytidine (5-Aza-CdR) on the proliferation of cervical cancer Hela cells and the expression of tumor suppressor genes CDH1 and HPV18E6 mRNA. Methods: Hela cells were treated with different concentrations of 5-Aza-CdR for 24 hours. The proliferation of Hela cells was detected by MTT assay. The expression of CDH1 and HPV18E6 mRNA was detected by semi-quantitative RT-PCR. Results: Compared with the control group, 5-Aza-CdR significantly inhibited Hela cell proliferation and showed a dose-response. 5-Aza-CdR could significantly inhibit the expression of CDH1 mRNA in Hela cells (P <0.05), but had no significant effect on the expression of HPV18E6 mRNA (P> 0.05). Conclusion: 5-Aza-CdR can inhibit the proliferation of Hela cells. The effect of 5-Aza-CdR may be through up-regulating the expression of CDH1, a tumor suppressor gene of cervical cancer, which may provide a new therapeutic approach for the treatment of cervical cancer.