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为了解蛙类在皖南山区携带钩端螺旋体(钩体)菌株的情况,安徽省疾病预防控制中心(安徽省疾控中心)于2002年在疫区捕捉蛙,取其肾分离培养,获得钩体23株。其中3株送中国药品生物制品检定所(检定所)检定。经血清学分类方法检定3株各不相同。现将检定结果报告如下。 1.材料与方法:3株菌分别为EG33株、EG79株和EG83株,均由安徽省疾控中心提供。实验用各群型钩体抗血清参考品、参考菌株均由检定所提供。各待检菌株的分群检定,首先与我国15群15型分群血清作凝集试验,根据待检菌株与相关分群血清交叉凝集滴度确定其所属菌群。如待检菌
In order to understand the situation of frogs carrying leptospira (leptospira) strains in the mountainous areas of southern Anhui, Anhui Provincial Center for Disease Control and Prevention (Anhui Provincial Center for Disease Control and Prevention) caught frogs in the affected areas in 2002, 23 strains. Three of them sent to China National Institute for the Control of Pharmaceutical and Biological Products (test institute). The serological classification of 3 strains vary. The test results are reported below. 1. Materials and Methods: The three strains were EG33, EG79 and EG83 respectively, all provided by CDC in Anhui Province. Experimental groups with hook antiserum reference materials, reference strains are provided by the test. The test of isolates of the isolates to be tested first agglutinated with 15 groups of 15 serogroups in our country, and determined the flora according to the cross titer of cross-linked serum of isolates to be tested. Such as bacteria to be checked