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目的研究TGF-β1基因-509位点C/T多态性与胃十二指肠疾病易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性方法,检测40例慢性胃炎,38例消化性溃疡,62例非贲门胃癌患者和102例正常对照者TGF-β1基因-509位点C/T等位基因及基因型分布,并对该基因多态性与幽门螺杆菌感染的相关性进行分析。结果 TGF-β1等位基因频率及基因型频率在病例组和对照组的分布无显著性差异;将胃十二指肠疾病患者按幽门螺杆菌感染分层后,结果提示TGF-β1基因型及等位基因频率分布无显著性差异。结论 TGF-β1-509位点基因多态性与胃十二指肠疾病幽门螺杆菌感染无关。
Objective To investigate the relationship between polymorphism of TGF-β1 gene at position -509 and susceptibility to gastroduodenal diseases. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect TGF-β1 gene -509 locus in 40 patients with chronic gastritis, 38 patients with peptic ulcer, 62 patients with non-cardiac gastric cancer and 102 normal controls. T allele and genotype distribution, and the correlation between the gene polymorphism and Helicobacter pylori infection were analyzed. Results There was no significant difference in the distribution of TGF-β1 allele frequency and genotype between case group and control group. After stratification of H. pylori infection in patients with gastroduodenal disease, the results suggest that TGF-β1 genotype and There was no significant difference in allele frequency distribution. Conclusion TGF-β1-509 locus polymorphism is not associated with H.pylori infection in gastroduodenal diseases.