[目的]观察舒胃汤对功能性消化不良(FD)肝郁脾虚型大鼠Cx43蛋白的分布及Cajal间质细胞的修复与再生的影响,探讨舒胃汤治疗FD的机制。[方法]将72只大鼠随机分为对照组、模型组、舒胃汤低剂量组(舒低组)、舒胃汤中剂量组(舒中组)、舒胃汤高剂量组(舒高组)和莫沙比利组(西药组),每组各12只。舒低组、舒中组、舒高组分别给予舒胃汤0.767g/ml、1.534g/ml、3.068g/ml,西药组予莫沙必利1.37mg/kg。采用复合病因造模(慢性束缚应激+过度疲劳+饮食失节),造成FD肝郁脾虚证大鼠模型。造模后第3天各组给予相应药液,对照组和模型组每日予以蒸馏水(10ml/kg),均为1次/d,持续14d。第15天处死取胃窦组织和小肠组织做免疫组织化学和荧光双染色观察Cx43蛋白的表达和ICC及神经纤维的形态。[结果]与对照组比较,模型组胃窦组织和小肠组织中Cx43蛋白阳性表达明显下降(P<0.01);与模型组比较,舒高组、舒中组和西药组胃窦组织和小肠组织中Cx43蛋白阳性表达明显升高(P<0.01);与对照组比较,模型组ICC超微结构损伤明显,胆碱能神经-ICC-SMC网络结构紊乱,ICC和神经纤维数目减少(P<0.01),荧光强度明显减弱(P<0.01);与模型组比较,舒高组、舒中组和西药组ICC超微结构较为正常完整,胆碱能神经-ICC-SMC网络基本完整,ICC和神经纤维数目明显增多(P<0.01),荧光强度明显加强(P<0.01)。[结论]舒胃汤能够上调Cx43蛋白的表达,修复ICC和促进ICC的再生,增加神经纤维的数目,从而保持胆碱能神经-ICC-SMC网络结构的完整,恢复胃肠动力而有效治疗FD。 [Objective] To observe the effect of Shuweitang on Cx43 protein distribution and repair and regeneration of Cajal interstitial cells in rats with functional dyspepsia (FD), and to explore the mechanism of Shuweitang in treating FD. [Methods] Seventy-two rats were randomly divided into control group, model group, Shuweitang low dose group (Shu Shu group), Shuweitang medium dose group (Shuzhong group) and Shuweitang high dose group Group) and mosapride group (western medicine group), 12 in each group. Shu Shu group, Shu high group, Shu high group were given Shu stomach soup 0.767g / ml, 1.534g / ml, 3.068g / ml, Western medicine group Mosapride 1.37mg / kg. The use of compound etiology modeling (chronic restraint stress + fatigue + eating disorders), resulting in FD rat model of liver spleen deficiency. On the 3rd day after modeling, each group was given the corresponding liquid, while the control group and model group were given distilled water (10ml / kg) daily for 1 time / d for 14 days. On the 15th day, the gastric sinus and small intestine were sacrificed and the expression of Cx43 protein and the morphology of ICC and nerve fiber were observed by immunohistochemistry and fluorescent double staining. [Results] Compared with the control group, the positive expression of Cx43 protein in gastric antrum and small intestine of model group was significantly decreased (P <0.01). Compared with model group, the expression of Cx43 protein in gastric antrum and small intestine in Shu Gao, Shu Zhong and western medicine groups (P <0.01). Compared with the control group, the ultrastructural damage of ICC in model group was obvious, the structure of cholinergic nerve-ICC-SMC network was disorganized, and the number of ICC and nerve fibers was decreased (P <0.01) (P <0.01). Compared with the model group, the ultrastructures of ICC in Shu Gao, Shu Zhong and Western Medicine groups were normal and complete, and the network of cholinergic nerve-ICC-SMC was basically intact. The ICC and nerve The number of fibers was significantly increased (P <0.01), and the fluorescence intensity was significantly enhanced (P <0.01). [Conclusion] Shuweitang can upregulate the expression of Cx43, repair ICC and promote the regeneration of ICC, increase the number of nerve fibers, so as to maintain the integrity of cholinergic nerve-ICC-SMC network structure and restore gastrointestinal motility and effectively treat FD .