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目的:探讨钒酸钠改善创伤后胰岛素抵抗和糖代谢紊乱的机制。方法:用钒酸钠胃饲30%TB-SAⅢ度烫伤大鼠,观察血糖、胰岛素、肌细胞膜胰岛素受体结合特性和胰岛素受体酪氨酸蛋白激酶(IR-TPK)活性的改变。结果:钒酸钠降低严重烫伤大鼠空腹血糖水平,不改变胰岛素水平,也不改变肌细胞膜胰岛素受体最大结合率和结合容量,但可显著增加胰岛素刺激的IR-TPK活性。结论:钒酸钠可不通过胰岛素受体介导而直接刺激IR-TPK活性,改善靶组织对胰岛素的敏感性。
Objective: To investigate the mechanism of sodium vanadate in improving post-traumatic insulin resistance and glucose metabolism disorder. Methods: Rats were sacrificed by intragastric administration of sodium vanadate (30% TB-SA) for 3 times, and the changes of insulin receptor binding and insulin receptor tyrosine kinase (IR-TPK) activity were observed. Results: Sodium vanadate decreased fasting plasma glucose (FBG) level in severely scalded rats, did not change the insulin level, and did not change the maximal binding rate and binding capacity of muscle cell membrane insulin receptor, but significantly increased insulin-stimulated IR-TPK activity. Conclusion: Sodium vanadate can directly stimulate the activity of IR-TPK without insulin receptor and improve the sensitivity of target tissue to insulin.