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将碱提类肝素(简称C)用阴离子交换树脂分离,其未吸附部分为组分1(C_1):吸附部分为组分2(C_2)。实验用195±SD25g体重的健康大鼠216只,??各半,随机分成3组。禁饲后心脏取血,三氯化铁法测定药前血清总胆固醇(totalcholesterol,TC)。次日ip 10％Triton 3ml/kg,1h后各组分别ip不同剂量的C,C_1和C_2,每个剂量12只大鼠。C组为400,300,200,100,50和0 mg/kg;C_1组800,600,400,100和0 mg/kg:C_2组300,200,100,50,25和0 mg/kg。各0剂量组均给予生理盐水(NS)4 ml/kg。8 h后再次测定血清TC;并按下式计算不同剂量的降脂率(D)。
The alkali-extracted heparin (referred to as C) was separated by an anion exchange resin and the unadsorbed part was component 1 (C 1): the adsorbed part was component 2 (C 2). A total of 216 healthy rats with 195 ± SD25g body weight were randomly divided into three groups. Blood was taken from the heart after feeding, and the total serum cholesterol (TC) was determined by the method of ferric chloride. The next day ip 10% Triton 3ml / kg, 1h after each group were ip different doses of C, C_1 and C_2, each dose of 12 rats. 400, 300, 200, 100, 50 and 0 mg / kg in group C, and 800, 600, 400, 100 and 0 mg / kg in group C_1: 300, 200, 100, 50, 25 and 0 mg / kg in C_2 group. Each 0 dose group was given normal saline (NS) 4 ml / kg. Serum TC was determined again after 8 h, and the lipid-lowering rate (D) at different doses was calculated according to the following formula.