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目的对幽门螺杆菌（Ｈｐ）的两种形态进行研究。方法采用细菌培养、电镜观察及ＰＣＲ扩增的ＤＮＡ多形性印迹分析等技术，观察幽门螺杆菌二种形态的转变。结果随着培养时间的延长，Ｈｐ逐渐由螺旋状体转变为圆球体。发生Ｌ型变异圆球体可分为二类，一类具有鞭毛且细胞膜完整，这一类圆球体可能具有活性；另一类圆球体系退行性变。Ｈｐ圆球体与螺旋状体的ＤＮＡ谱十分相似，并且两者都分泌过氧化氢酶、碱性磷酸酶及酸性磷酸酶。结论Ｈｐ圆球体仍具有螺旋状体的诸多生物学共性，至少部分圆球体可能具有活性。因而，在Ｈｐ的传播及相关疾病的治疗复发过程中起重要作用。
Objective To study two forms of Helicobacter pylori (Hp). Methods Bacterial culture, electron microscopy and PCR amplification of DNA plethysmography were used to observe the morphological changes of Helicobacter pylori. Results With the extension of culture time, Hp gradually transformed from spheroid to spheroid. Occurrence of L-shaped variation of the sphere can be divided into two categories, one with flagella and the integrity of the cell membrane, this type of sphere may be active; another type of ball system degeneration. The Hp sphere has a very similar DNA profile to that of the helix, and both secrete catalase, alkaline phosphatase and acid phosphatase. Conclusions Hp spheroids still have many biological universals of helical bodies, and at least part of them may be active. Therefore, Hp transmission and related diseases play an important role in the process of relapse.