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细菌性果斑病是全世界范围内瓜类生产中的重要病害,其病原菌是西瓜噬酸菌(Acidovorax citrulli).细菌Tpye-IV菌毛的生物合成及功能由十几个基因参与调控,pilO是其中的一个基因.运用同源重组的方法,构建了西瓜噬酸菌AAC-3菌株Type IV菌毛结构蛋白编码基因pilO的功能缺失突变体菌株ΔpilO,对突变体的生长能力、运动能力、生物薄膜形成能力及致病力等进行了测定,同时对菌体形态进行观察.透射电镜观察结果显示突变体菌株不能形成菌毛.ΔpilO突变体菌株的生长基本不受影响,但是其运动能力显著下降,生物薄膜形成能力降低80%,致病力与野生型菌株相比下降了55%.本研究结果表明pilO基因在病原菌Type IV菌毛形成及其运动性和致病力等方面发挥重要作用.
Bacterial fruit spot is an important disease in melon production in the world.The pathogen is Acidovorax citrulli.The biosynthesis and function of bacterial Tpye-IV pilus are controlled by a dozen genes, and pilO Is one of the genes.Using homologous recombination, we constructed ΔpilO, a function deletion mutant of PilO of type IV pilus structural protein of Watermelon phagerae AAC-3 strain, and evaluated the growth, movement, Biofilm formation ability and virulence were measured, at the same time, the morphology of the cells was observed.Transmission electron microscopy showed that the mutant strain could not form pili.The growth ofΔpilO mutant strain was basically unaffected, but its exercise capacity was significant Decreased, biofilm formation ability decreased by 80%, and pathogenicity decreased by 55% compared with wild-type strain.The results of this study indicate that pilO gene plays an important role in the formation of pilus in Type IV pathogen and its motility and pathogenicity .