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目的:探讨E-选择素及其配体SLeX在大肠癌LoVo细胞与血管内皮细胞ECV304早期黏附中的作用。方法:采用SP法观察E-选择素在内皮细胞ECV304及LoVo中的表达;采用活性染料玫瑰红摄入法检测E-选择素及其配体SLeX在大肠癌LoVo细胞与TNF-α激活内皮细胞ECV304早期黏附中作用。结果:E-选择素和SLeX分别在ECV304和LoVo细胞膜及细胞质内有明确的阳性表达;血管内皮细胞ECV304被TNF-α激活后,其与LoVo细胞间的黏附较激活前明显增加,且具有浓度及时间的依赖性,P<0.001;用不同浓度的抗E-选择素单抗处理血管内皮细胞ECV304,或用其配体SLeX单抗处理肿瘤Lo-Vo细胞后,随着E-选择素单抗、SLeX单抗浓度增加,细胞相对黏附率逐渐降低(P<0.001),阻断率逐渐增加(P均<0.05),提示E-选择素单抗、SLeX单抗可阻断大肠癌细胞与血管内皮细胞间黏附。结论:E-选择素和其配体SLeX是介导血管内皮细胞与大肠癌Lo-Vo细胞系黏附反应的主要早期黏附分子。
Objective: To investigate the role of E-selectin and its ligand SLeX in the early adhesion of colorectal cancer LoVo cells and vascular endothelial cells ECV304. Methods: The expression of E-selectin in ECV304 and LoVo cells was detected by SP method. The expression of E-selectin and its ligand SLeX in colorectal cancer LoVo cells and TNF-α-activated endothelial cells ECV304 early adhesion role. Results: The positive expression of E-selectin and SLeX in the membrane and cytoplasm of ECV304 and LoVo, respectively. After activated by TNF-α, the expression of E-selectin and LoVo in ECV304 cells was significantly increased compared with LoVo cells, And time dependent, P <0.001. After treated with different concentrations of anti-E-selectin monoclonal antibody (ECV304) or tumor-bearing Lo-Vo cells with its ligand SLeX monoclonal antibody, (P <0.001), and the blocking rate increased gradually (P <0.05), suggesting that E-selectin monoclonal antibody and SLeX monoclonal antibody can block the colorectal cancer cells and Vascular endothelial cell adhesion. CONCLUSION: E-selectin and its ligand SLeX are the major early adhesion molecules that mediate the adhesion of vascular endothelial cells to colorectal cancer Lo-Vo cell line.