耐热ＤＮＡ聚合酶在大肠杆菌中得到表达。在实验室表达、鉴定分析的基础上，进行了中试制备及鉴定。１０批酶的中试结果表明，该工程菌复活率达到１００％，表达性能稳定，每１００ｍｌ细菌培养物产酶平均达到１．７６×１０６Ｕ，比活平均达到２．６２×１０４Ｕ／ｍｇ蛋白。经初步应用，能够催化ＰＣＲ扩增反应。 Thermostable DNA polymerase is expressed in E. coli. Based on the laboratory expression, identification and analysis, the pilot plant preparation and identification were carried out. The results of ten batches of experiments showed that the recovery rate of the engineered bacteria reached 100%, and the expression performance was stable. The average yield of enzyme per 100 ml of bacterial culture reached 1.76 × 106 U, and the specific activity averaged 2.62 × 104 U / mg protein. After preliminary application, it can catalyze PCR amplification reaction.