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目的 探讨膀胱癌患者尿脱落细胞中细胞角蛋白 2 0 (CK2 0 )的表达及临床意义。 方法 采用RT PCR方法检测 4 1例膀胱癌、2 0例其他泌尿系疾病患者及 10例正常人尿脱落细胞中CK2 0的表达。 结果 10例正常人的尿脱落细胞均未表达CK2 0 ;2 0例非膀胱癌者中 3例表达CK2 0 ;4 1例膀胱癌患者中 37例表达CK2 0 ,三组间比较差别有显著性意义 (P <0 .0 1)。G1、G2 及G3膀胱癌的阳性率分别为 90 .2 % (2 3/2 5 )、88.8% (8/9)及 85 .7% (6 /7) ,三组比较差别无显著性意义(P >0 .0 5 )。 结论 CK2 0在膀胱癌患者尿脱落细胞中表达阳性率高 ,可用于膀胱癌的诊断及术后随访。
Objective To investigate the expression and clinical significance of cytokeratin 20 (CK20) in urine exfoliated cells of patients with bladder cancer. Methods The expression of CK20 in 4 1 bladder cancer, 20 urinary tract diseases and 10 normal urine exfoliated cells were detected by RT-PCR. Results None of the 10 normal human urine exfoliated cells expressed CK2 0; 3 of 20 non-bladder cancer expressed CK2 0; 4 of 37 bladder cancer patients expressed CK2 0, the difference between the three groups was significant Significance (P <0 .01). The positive rates of G1, G2 and G3 in bladder cancer were 90.2% (2 3/2 5), 88.8% (8/9) and 85.7% (6/7), respectively. There was no significant difference between the three groups (P> 0.05). Conclusion The positive rate of CK20 expression in urinary exfoliated cells in patients with bladder cancer is high, which can be used in the diagnosis of bladder cancer and follow-up.