论文部分内容阅读
[目的]克隆梅毒螺旋体(Treponema pallidum)蛋氨酸亚枫还原酶(methionine sulfoxide reductase,Msr)基因并对其进行生物分信息学分析。[方法]根据梅毒螺旋体Msr基因序列,设计特异性引物,PCR法扩增目的基因,将其连接到p MD19-T载体,进行DNA测序及生物信息学分析。[结果]从梅毒螺旋体中扩增得到的大小为873 bp的Msr基因,编码291个氨基酸。Msr蛋白含有Msr A和Msr B的保守区,为亲水性蛋白,无信号肽,定位在细菌细胞质。三维结构与牛Msr蛋白(PDB:1fvg.1)类似。Msr蛋白含有几个B表位。[结论]克隆获得了Tp Msr基因,并分析了其生物学特性。
[Objective] The study aimed to clone the methionine sulfoxide reductase (Msr) gene of Treponema pallidum and analyze it by bioinformatics. [Method] According to the sequence of Msr gene of Treponema pallidum, specific primers were designed. The target gene was amplified by PCR and ligated into pMD19-T vector for DNA sequencing and bioinformatics analysis. [Result] The 873 bp Msr gene was amplified from Treponema pallidum and encoded 291 amino acids. The Msr protein contains conserved regions of Msr A and Msr B, which are hydrophilic proteins with no signal peptide and are localized in the bacterial cytoplasm. The three-dimensional structure is similar to the bovine Msr protein (PDB: 1fvg.1). The Msr protein contains several B epitopes. [Conclusion] The Tp Msr gene was cloned and its biological characteristics were analyzed.