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目的研究17β雌二醇(E2)对大鼠血管平滑肌细胞(VSMC)生长的影响。方法应用流式细胞仪检测不同浓度E2在有或无血清刺激下对传代VSMC细胞周期及其相关蛋白Cy-clinD1和CDK4表达的影响。结果在有血清的条件下,E2(1、10、100nmol/L)促进VSMC从G1期向S过渡,其S期细胞比率分别为(31.89±9.14)%、(35.90±4.59)%、(30.77±1.20)%,显著高于对照细胞(21.63±1.80)%(P<0.05),并伴随CyclinD1和CDK4蛋白表达量的显著增高;而在无血清的环境中,高浓度(10、100nmol/L)E2则抑制VSMC增殖,其S期细胞比率分别为(9.93±1.43)%、(8.76±1.80)%,显著低于对照细胞(16.58±3.04)%(P<0.05),且伴随Cy-clinD1和CDK4蛋白表达量降低。结论E2在有或无血清条件下分别对VSMC增殖起促进或阻滞作用,其机制可能是通过影响CyclinD1和CDK4蛋白的表达而作用于VSMCG1期。
Objective To study the effect of 17β estradiol (E2) on the growth of rat vascular smooth muscle cells (VSMCs). Methods Flow cytometry was used to detect the effects of different concentrations of E2 on the cell cycle and the expression of Cy-clinD1 and CDK4 in VSMCs with or without serum stimulation. Results E2 (1, 10, 100 nmol / L) promoted the transition of VSMC from G1 phase to S phase under the condition of serum. The S phase cell ratio was (31.89 ± 9.14)%, (35.90 ± 4.59)%, ± 1.20)%, which was significantly higher than that of the control cells (21.63 ± 1.80)% (P <0.05), accompanied by a significant increase of the expression of CyclinD1 and CDK4 protein. In the serum-free environment, ) E2 inhibited the proliferation of VSMC, and the percentage of S phase cells was (9.93 ± 1.43)% and (8.76 ± 1.80)% respectively, which was significantly lower than that of control cells (16.58 ± 3.04)% And CDK4 protein expression decreased. Conclusion E2 can promote or retard the proliferation of VSMC in the presence or absence of serum, respectively. The possible mechanism is that E2 may affect the expression of CyclinD1 and CDK4 in VSMCG1.