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目的 观察抗氧化干预在粒细胞减少大鼠肺部铜绿假单胞菌感染中的治疗作用。方法 雄性SD大鼠 90只 ,分为 2组 ,A组 :ICH对照组 ,制作粒细胞减少模型 ;B组 :抗氧化干预组 ,先按A组方法制作粒细胞减少模型 ,半小时后腹腔注射N 乙酰半胱氨酸 (NAC) 15 0mg·kg-1·d-1,连续 7d ,接种细菌前半小时再注射 1次NAC。用铜绿假单胞菌标准菌株制作肺部感染模型。观察感染 0h、6h、9h、2 4h、4 8h和 72h的外周血白细胞、氧化 /抗氧化指标、肺组织细菌负荷、肺血管渗透性指标和肺湿干比 (W/D)、肺组织病理学改变等。结果 2组大鼠感染前外周血白细胞均低于 4×10 9/L ,B组血清和肺组织中丙二醛水平明显低于A组 ,2组肺组织细菌负荷无明显差异 ,B组肺血管渗透性和肺W/D均明显低于A组 ,而超氧化物歧化酶水平明显高于A组。肺组织病理学显示 ,A组肺损伤严重 ,肺充血 ,出血明显 ,肺上皮细胞凋亡小体形成。结论 粒细胞减少大鼠肺部感染时抗氧化干预可减轻肺损伤 ,抗氧化治疗可能成为粒细胞减少肺部感染的重要辅助治疗手段之一
Objective To observe the therapeutic effect of anti-oxidative intervention in pulmonary Pseudomonas aeruginosa infection in rats with granulocytopenia. Methods 90 male Sprague-Dawley rats were divided into 2 groups. Group A: ICH control group, making model of granulocytopenia; Group B: anti-oxidative intervention group, first making a model of granulocytopenia according to group A. Half an hour later, intraperitoneal injection N acetylcysteine (NAC) 15 0 mg · kg-1 · d-1 for 7 days. NAC was injected once more in the first half hour after inoculation of bacteria. Pulmonary infection models were made with standard strains of Pseudomonas aeruginosa. Peripheral leukocytes were observed at 0h, 6h, 9h, 24h, 48h and 72h after infection. Bacterial load, lung vascular permeability index, lung wet / dry ratio (W / D) Neo-Confucianism changes. Results The levels of peripheral blood leukocytes in the two groups before infection were lower than 4 × 10 9 / L, while the levels of malondialdehyde in serum and lung tissue of group B were significantly lower than those in group A, while there was no significant difference in bacterial load between group 2 and group B Vascular permeability and lung W / D were significantly lower than in group A, while the level of superoxide dismutase was significantly higher than that of group A. Lung histopathology showed severe lung injury in group A, pulmonary congestion and hemorrhage, and formation of apoptotic bodies in lung epithelial cells. Conclusions Anti-oxidant intervention can reduce lung injury in lungs of granulocytopenic rats and anti-oxidative therapy may be one of the important adjuvant therapy for the treatment of granulocytopenia pulmonary infection