论文部分内容阅读
目的调查肺癌组织中结核分枝杆菌L-型的感染状况,以分析结核分枝杆菌L-型感染相关肺癌的临床病理学特征。方法采用原位分子杂交技术检测结核分枝杆菌L-型MPB64基因片段的存在及定位。同时,用IK抗酸染色法检测标本中的结核分枝杆菌L-型。结果原位分子杂交检测石蜡包埋73例肺癌组织中MPB64基因片段在肺鳞癌、肺腺癌、细支气管肺泡癌、小细胞未分化癌中的阳性率分别为85.0%、73.5%、81.8%和87.5%,差异无统计学意义(P>0.05)。IK抗酸染色检测结核分枝杆菌L-型在肺鳞癌、肺腺癌、细支气管肺泡癌、小细胞未分化癌中的阳性率分别为70.0%、64.7%、63.6%和75.0%,差异无统计学意义(P>0.05)。结论结核分枝杆菌L-型感染在肺癌的形成过程中可能起到一定的作用,但是与癌组织的病理学类型无关。
Objective To investigate the infection status of L-form of Mycobacterium tuberculosis in lung cancer tissues and to analyze the clinicopathological features of L-type infection-associated lung cancer of Mycobacterium tuberculosis. Methods The presence and location of the Mycobacterium tuberculosis L-type MPB64 gene fragment was detected by in situ hybridization. At the same time, IK antacid staining was used to detect the M. tuberculosis L-form. Results In situ hybridization detection of paraffin-embedded MPB64 gene fragment in 73 cases of lung cancer tissues in lung squamous cell carcinoma, lung adenocarcinoma, bronchioloalveolar carcinoma, small cell undifferentiated carcinoma of the positive rates were 85.0%, 73.5%, 81.8% And 87.5%, the difference was not statistically significant (P> 0.05). The positive rates of mycobacterium tuberculosis L-type detection by IK acid-fast staining in lung squamous cell carcinoma, lung adenocarcinoma, bronchioloalveolar carcinoma and small cell undifferentiated carcinoma were 70.0%, 64.7%, 63.6% and 75.0%, respectively No statistical significance (P> 0.05). Conclusion L-type infection of Mycobacterium tuberculosis may play a role in the formation of lung cancer, but not with the pathological type of cancer.