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为了解犬牙僵虾虎鱼体内河豚毒素毒性的动态变化,于2012年1月至12月,对湛江港地区犬牙僵虾虎鱼毒性变化进行了一周年的跟踪监测,利用质谱定量检测其体内的毒力,研究其毒性变化规律。实验结果表明:犬牙僵虾虎鱼体内河豚毒素毒力呈季节性变化,每年的春、秋季毒力较高,其中春季3月是全年毒力最高的月份,整鱼毒力达0.300×10-6。对犬牙僵虾虎鱼各部位毒力作进一步分析表明,河豚毒素在犬牙僵虾虎鱼各部位的积蓄程度不一,3月犬牙僵虾虎鱼河豚毒素主要积蓄于性腺,毒力达0.433×10-6,其次为鱼肝、鱼头,毒力最低的部位为鱼身;7月犬牙僵虾虎鱼毒素主要积蓄于鱼皮,其次为鱼肝、鱼头,毒力最低的部位仍为鱼身;3月、4月及6月至10月河豚毒素在鱼头的积蓄明显高于鱼身,毒力最高时期的3月鱼头毒力达0.356×10-6,2月和11月河豚毒素在鱼身的积蓄高于鱼头,2月鱼身毒力达0.325×10-6。
In order to understand the dynamic changes of tetrodotoxin toxicity in canine tooth-goby, from January to December in 2012, the first year of follow-up monitoring was carried out on the toxic changes of canine stomatal goby in Zhanjiang harbor area. Quantitative detection of in vivo Toxicity, to study the changes of its toxicity. The experimental results showed that the virulence of tetrodotoxin in canine tooth-goby had seasonal changes, with higher virulence in spring and autumn each year, of which the highest virulence in March was in March and the virulence in whole fish reached 0.300 × 10 -6. Further analysis of the virulence of various parts of the gnathostatus goby showed that the accumulative levels of tetrodotoxin in various parts of the gnathostatus varied from March to March. The gazelle toxic goby mainly accumulated in the gonads in March, with a virulence of 0.433 × 10 -6, followed by liver, head, virulent parts of the body of the fish; July canine tooth stinging tiger toxins are mainly deposited in the skin, followed by the liver, head, the lowest virulence sites are still fish Body; March, April and June to October tetrodotoxin savings in fish head was significantly higher than the fish body, the highest virulence of March head toxicity reached 0. 356 × 10 - 6, in February and November puffer fish Toxins in fish body savings higher than the head of fish in February virulence up to 0.325 × 10-6.