木耳是一种胶质菌,耳片附着有大量的杂菌,采用一般消毒方法很难把杂菌彻底杀死,即使在培养基中添加抗菌素亦难于凑效。所以,采用耳片组织分离法培育木耳纯菌丝体的成功率很低。经实验,我们作如下改进,效果较好。方法是现场采摘的耳片,在阴凉处稍晾干,用流水反复冲洗后,放入0.1%升汞水中浸泡一分种。然后用无菌水反复冲洗耳片,除去药液,用灭菌干纱布吸干耳片,将其放入灭菌的培养皿内。在无菌条件下,将耳片斜切成两块,用锋利的接种 Edible fungus is a kind of gum bacteria, the ears attached a large number of bacteria, the general sterilization method is difficult to completely kill the bacteria, even in the medium to add antibiotics also difficult to work together. Therefore, the use of ear tissue isolation culture fungus mycelium success rate is very low. After experiments, we make the following improvements, the effect is better. The method is picking the ears of the scene, a little dry in the shade, rinsed with water repeatedly, into 0.1% Mercury in water to soak a sub-species. Then rinse the ear repeatedly with sterile water, remove the liquid, dry the ear with sterilized dry gauze, place it in a sterile Petri dish. Under aseptic conditions, the beaks were beveled in two pieces and sharply inoculated