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用双波长、差示、一阶导数分光光度法，建立了一种测定外源物体外代谢时一氧化碳（ＣＯ）生成量的新方法。与传统的血红蛋白ＣＯ络合物生成测定ＣＯ法相比，本方法能有效地消除背景干扰、提高ＣＯ定量的准确性与灵敏度。利用所建立的方法。验证了四种三卤代苯胺和一种三卤代苯经鼠肝微粒体代谢生成ＣＯ的能力。其中，仅２，４，５－三氟苯胺（２，４，５－ＴＦＡ）可生成较大量的ＣＯ。尽管２，４，６－三氟苯胺代谢脱氟的速度与２，４，５－ＴＦＡ相近，但生成ＣＯ的能力仅为后者的１／１０左右。用苯巴比妥或地塞米松诱导大鼠，可明显提高鼠肝微粒体使２，４，５－ＴＦＡ代谢生成ＣＯ的能力，对其余化合物无类似影响。上述观察进一步证明了此生成ＣＯ的降解途径，很可能是某类Ｐ４５０同功酶的特异性反应，同时还提示，此过程亦具有底物特异性。
A new method for determining the amount of carbon monoxide (CO) produced by exogenous extracellular metabolites was established by dual wavelength, differential, first-order derivative spectrophotometry. Compared with the traditional method of measuring hemoglobin CO complex formation CO, the method can effectively eliminate background interference and improve the accuracy and sensitivity of CO quantification. Using the established method. The ability of four trihaloaniline and one trihalobenzene to metabolize CO by rat liver microsomes was verified. Among them, only 2,4,5-trifluoroaniline (2,4,5-TFA) can generate a larger amount of CO. Although the 2,4,6-trifluoroaniline metabolizes defluoridation at a rate similar to 2,4,5-TFA, its ability to generate CO is only about 1/10 of the latter. Induction of rats with phenobarbital or dexamethasone significantly increased the ability of rat liver microsomes to metabolize 2,4,5-TFA to CO, with no similar effect on the remaining compounds. This observation further demonstrates that this generation of CO degradation pathway is likely to be a specific reaction of certain P450 isoenzymes and also suggests that this process is also substrate specific.