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目的建立同时检测麻仁丸中厚朴酚、和厚朴酚、大黄素及大黄酚的测定方法。方法采用HPLC法。C18色谱柱;甲醇-0.1%磷酸水溶液为流动相,进行梯度洗脱;体积流量1.0mL/min;检测波长254nm。结果根据回归方程,4种成分线性范围:和厚朴酚6.285～62.85μg/mL,R2=0.9996;厚朴酚14.57～145.7μg/mL,R2=0.9996;大黄素2.744～27.44μg/mL,R2=0.9993;大黄酚6.828～68.28μg/mL,R2=0.9992;平均回收率:和厚朴酚98.8%,RSD为1.0%(n=6),厚朴酚99.9%,RSD为1.4%(n=6),大黄素98.9%,RSD为2.0%(n=6),大黄酚98.4%,RSD为1.6%(n=6)。结论该方法操作简便、准确,重复性好,可用于麻仁丸的质量控制。
Objective To establish a method for simultaneous determination of magnolol, honokiol, emodin and chrysophanol in Maren pill. Methods HPLC method. C18 column; methanol-0.1% phosphoric acid aqueous solution as the mobile phase, gradient elution; volume flow 1.0mL / min; detection wavelength 254nm. Results According to the regression equation, the linear ranges of the four components were as follows: honokiol 6.285-62.85μg / mL, R2 = 0.9996; honokiol 14.57-145.7μg / mL, R2 = 0.9996; emodin 2.744-27.44μg / mL R2 (N = 6), honokiol 99.9%, and RSD 1.4% (n = 6). The average recoveries of honokiol were 98.8%, RSD was 1.0% 6), emodin 98.9%, RSD 2.0% (n = 6), chrysophanol 98.4%, RSD 1.6% (n = 6). Conclusion The method is simple, accurate, reproducible and can be used for the quality control of Maren pill.