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目的 了解胶体金法检测O1群霍乱弧菌的特异性、灵敏度和实用性.方法 取患者粪便标本,先用碱性蛋白胨水35℃增菌6 h,然后用胶体金法进行检测,同时作霍乱弧菌常规培养,培养出细菌后用血清凝集法作为鉴别诊断O1群霍乱弧菌的金标准,用统计学方法对胶体金法诊断试验的评价指标进行计算..结果 700份样本中,常规法培养出O1霍乱弧菌105株,胶体金法试验阳性108株,经χ2检验,差异无统计学意义(χ2=0.24,P>0.05),表明胶体金法检测O1群霍乱弧菌与常规培养法检测结果之间的差异无统计学意义.胶体金法检测O1群霍乱弧菌诊塑试验的灵敏度为93.33%,特异性为98.32%,阳性预测值为90.74%,阴性预测值为97.18%,假阴性率为1.68%,假阳性率为6.67%.结论 胶体金法检测O1群霍乱弧菌特异性高(98.32%),灵敏度好,与常规培养法的一致性也较高,在快速检测O1群霍乱弧菌中有一定应用价值.“,”Objective To understand the specificity, sensitivity and practicality of colloidal gold assay in the detection of Vibrio cholerae Ol group. Methods Stool specimens of patients were incubated in alkaline peptone water (35℃) for 6 hours. Then the colloidal gold method was used to detect Vibrio cholerae. At the same time conventional culture of Vibrio cholerae was identified with serum agglutination as the gold standard. The results of two methods were compared. Results Among 700 samples,105 Vibrio cholerae were identified with conventional methol method and 108 identified with coilidal gold assay and there was no significant difference between the two methods. (χ2 = 0.24 ,P > 0.05). The sensitivity of col-loidal gold method for Vibrio cholerae O1 detetion was 93.33% ,with a specificity of 98.32 ,a positive predictive value of 90. 74% ,a negative value of 97.18% ,a false negative rate of 1.68% ,and a false positive rate of 6.67%. Conclusion The colloidal gold method has good specificity and sensiticity and consistency with conventional method in Vibrio cholerae Ol detection. The method is value.able in rapid detection of Vibrio chplerae O1.