无菌取5～6周龄C57BL Kaplan 小鼠胸腺,经胶原酶消化,低密度细胞分层,据其粘附性的不同分离提取树突状细胞,纯度为90%以上。以流式细胞仪检测树突状细胞的表面标志。结果表明,树突状细胞表达高 The thymus of C57BL Kaplan mice, aged 5-6 weeks old, was aseptically digested with collagenase and dendritic cells were decellularized. Dendritic cells were isolated and separated according to their adhesiveness with a purity of 90%. Dendritic cell surface markers were detected by flow cytometry. The results show that dendritic cells are highly expressed