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目的:利用高效毛细管电泳-场强放大柱内堆积技术分离测定白花丹参中的水溶性有效成分原儿茶醛,原儿茶酸,丹参素,迷迭香酸和丹酚酸B。方法:采用未涂层熔融石英毛细管柱(75μm×50.2 cm,40 cm),以含15%甲醇的20 mmol.L-1硼砂(pH 10.0)为运行缓冲液,分离电压28 kV,柱温25℃,检测波长210 nm。进样前压力进水3.4 kPa;电动进样-8kV×3 s。结果:原儿茶醛的线性范围是3.0～60.0 mg.L-1(R2=0.999 6);原儿茶酸,丹参素,迷迭香酸,丹酚酸B的线性范围均为1.0～20.0 mg.L-1(R2=0.999 1,0.999 4,0.998 9,0.999 8),检测限分别为0.55,0.40,0.25,0.32,0.38μg.L-1,富集倍数高,灵敏度高;将此方法应用于实际样品测定,回收率为97.31%～99.81%。结论:该方法简单、快速、准确、灵敏度高,可用于白花丹参的质量控制。
OBJECTIVE: To isolate and determine the water-soluble active ingredients protocatechuic aldehyde, protocatechuic acid, danshensu, rosmarinic acid and salvianolic acid B in Salvia miltiorrhiza by high performance capillary electrophoresis-field amplification column packing technique. METHODS: Uncoated fused silica capillary columns (75 μm × 50.2 cm, 40 cm) were used as running buffer with 20 mmol·L-1 borax (pH 10.0) containing 15% ℃, detection wavelength 210 nm. Before injection pressure water 3.4 kPa; Electric injection -8 kV × 3 s. Results: The linear range of protocatechuic aldehyde was 3.0-60.0 mg.L-1 (R2 = 0.999 6). The linear ranges of protocatechuic acid, danshensu, rosmarinic acid and salvianolic acid B were 1.0-20.0 The detection limits were 0.55,0.40,0.25,0.32 and 0.38μg.L-1, respectively, with high enrichment factor and high sensitivity. The method was applied to the determination of the actual sample, the recovery rate was 97.31% ~ 99.81%. Conclusion: The method is simple, rapid, accurate and sensitive and can be used for the quality control of Salvia miltiorrhiza.