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目的:探讨胎盘组织中KiSS-1mRNA及其蛋白肽metastin表达在早发重度子癎前期发病中的作用。方法:采用RT-PCR、Western blot、免疫组化方法检测57例子癎前期患者(轻度15例和重度42例,其中早发重度12例,晚发重度30例)和30例正常晚期妊娠者胎盘组织中KiSS-1mRNA及其蛋白肽metastin的表达水平和定位。结果:胎盘组织中蛋白肽metastin主要表达在绒毛的合体滋养细胞和细胞滋养细胞。子癎前期组胎盘组织中KiSS-1mRNA及蛋白肽metastin表达分别为(1.73±0.24)和(78.41±7.96)μg/100μg总蛋白,显著高于正常晚期妊娠组(P均<0.01),以早发重度子癎前期组尤为显著。结论:子癎前期存在KiSS-1mRNA及其蛋白肽metastin表达增强,且早发重度子癎前期组表达显著增高,可能是早发重度子癎前期发病的主要原因之一。
Objective: To investigate the role of KiSS-1 mRNA and its protein peptide metastin in the pathogenesis of preeclampsia of placenta previa. Methods: 57 cases of preeclampsia (mild 15 cases and severe 42 cases, including 12 cases of early onset severe and 30 cases of severe late onset) and 30 cases of normal late pregnancy were detected by RT-PCR, Western blot and immunohistochemistry The expression level and localization of KiSS-1 mRNA and its protein peptide metastin in placenta tissue. RESULTS: The placental protein peptide metastin was mainly expressed in villous syncytiotrophoblasts and cytotrophoblasts. The expression of KiSS-1mRNA and protein peptide metastin in pre-fetus placenta were (1.73 ± 0.24) and (78.41 ± 7.96) μg / 100μg total protein, respectively, which were significantly higher than those in normal late pregnancy (P <0.01) Severe sub-发 pre-term group is particularly significant. CONCLUSIONS: The expression of KiSS-1 mRNA and its protein peptide metastin in preeclampsia is increased, and the expression of KiSS-1 in early preeclampsia and preeclampsia is significantly increased, which may be one of the major causes of early onset of severe preeclampsia.