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目的观察青藤碱对DC2.4树突状细胞的生物学活性的影响。方法培养DC2.4细胞,根据MTT方法确定药物最佳给药浓度及给药时间,分为正常组、脂多糖(LPS)模型对照组、40μg/m L青藤碱高浓度组、20μg/m L青藤碱组、10μg/m L青藤碱组、25 ng/m L甲氨蝶呤阳性对照组。5μg/m L LPS刺激DC2.4细胞24 h,给予不同浓度的青藤碱,刺激24、48、72 h,收集细胞上清液,ELISA检测细胞上清液中的γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、IL-10、IL-12、CD80的含量。结果不同浓度青藤碱处理各组与甲氨蝶呤处理组均可降低TNF-α、CD80、IFN-γ、IL-6、IL-12的含量,而各处理组的IL-10含量均有所增加,且20μg/m L终浓度青藤碱作用后的TNF-α、CD80、IFN-γ、IL-6、IL-10、IL-12的含量相比于其他浓度青藤碱更接近正常组的含量。结论 20μg/m L青藤碱可抑制DC2.4细胞的生物学活性,减少DC的炎症因子分泌。
Objective To observe the effect of sinomenine on the biological activity of DC2.4 dendritic cells. Methods DC2.4 cells were cultured and the optimal drug concentration and administration time were determined according to MTT method. The cells were divided into normal group, LPS model control group, 40μg / ml sinomenine high concentration group, 20μg / m L sinomenine group, sinomenine 10μg / m L group, methotrexate 25 ng / m L positive control group. DC2.4 cells were stimulated with 5μg / mL LPS for 24 hours, then treated with different concentration of sinomenine for 24h, 48h and 72h. Cell supernatants were collected and IFN-γ ), Tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), IL-10, IL-12 and CD80 were detected. Results Sinomenine treatment with different concentrations of methotrexate treatment group can reduce the content of TNF-α, CD80, IFN-γ, IL-6, IL-12, , And the levels of TNF-α, CD80, IFN-γ, IL-6, IL-10 and IL-12 after sinomenine treatment at a final concentration of 20 μg / mL were closer to normal than that of sinomenine at other concentrations Group content. Conclusion Sinomenine 20μg / ml can inhibit the biological activity of DC2.4 cells and reduce the secretion of inflammatory cytokines.