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本文采用几种不同类型的弱碱阴离子交换树脂对谷氨酸、天门冬氨酸和苏氨酸的分离性能进行了研究,考察了树脂结构、淋洗剂和树脂交换基团的型式对分离性能的影响。结果发现以pH~4缓冲溶液处理树脂,以醋酸,盐酸溶液分部淋洗两种酸性氨基酸得到完全分离。
In this paper, the separation performance of glutamic acid, aspartic acid and threonine with several different kinds of weak base anion exchange resins was studied. The effects of the type of resin structure, eluent and resin exchange group on the separation performance Impact. The results showed that the resin was treated with pH ~ 4 buffer and the two acidic amino acids were eluted completely with acetic acid and hydrochloric acid.