组织化学中可应用二氨基联苯胺(DAB)法显示红细胞的过氧化氧化物酶,但应用DAB法显示微血管则在国内尚未见报道。我们经过多次摸索,终于应用DAB法显示微血管获得成功,并首次应用此法对大鼠、兔、猫等的脑、肝、肾、肠等器官进行试验,均取得了满意的效果。此法的操作过程是:动物快速断头或经麻醉后取材,固定于Baker福尔马林或含10%福尔马林的0.1 M磷酸缓冲液(pH 7.6)中1～数天。冰冻切片,厚100～300微米。切片入0.05%DAB液 Diamino benzidine (DAB) method can be used to display red blood cell peroxidase in histochemistry. However, the use of DAB method to display microvascular has not been reported in China. After repeated exploration, we finally obtained the satisfactory results by using the DAB method to demonstrate the success of capillaries, and for the first time using this method to test the brain, liver, kidney, intestine and other organs of rats, rabbits and cats. The procedure is as follows: animals are decapitated or anesthetized and fixed in Baker formalin or 0.1 M phosphate buffer (pH 7.6) containing 10% formalin for 1 to several days. Frozen section, thickness 100 ~ 300 microns. Slice into 0.05% DAB solution