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目的:对复方脑忆源片中主要成分淫羊藿苷进行转运研究,阐明吸收特点。方法:利用Caco-2细胞模型,对复方脑忆源片中主要成分淫羊藿苷(ICA)进行体外转运,并通过高效液相(Luna Kromasil C18色谱柱(4.6 mm×150 mm,5μm);流动相乙腈∶水(30∶70);流速1.0 mL.min-1;检测波长270 nm;)测定单体、药材及复方中的淫羊藿苷转运后的含量。结果:淫羊藿苷在小肠吸收转运过程随时间增长而增加,可能会被Caco-2细胞中的P-gp转运蛋白外排。与淫羊藿单体和淫羊藿提取物中的淫羊藿苷比较,脑忆源片可显著提高淫羊藿苷的转运,同时可降低淫羊藿苷的外排作用。结论:在脑忆源片中,由于淫羊藿苷外排减少,可认为通过复方配伍后,方中的其他药物抑制了淫羊藿苷的外排,使其可以更好的发挥药理作用。
OBJECTIVE: To study the translocation of icariin, the main component of compound brain-memory film, to elucidate the characteristics of absorption. Methods: Icariin (ICA), the main component of Compound Meixian Tablets, was transfused in vitro using Caco-2 cell model and was separated on a Luna Kromasil C18 column (4.6 mm × 150 mm, 5 μm) Mobile phase acetonitrile: water (30:70); flow rate 1.0 mL.min-1; detection wavelength 270 nm;) determination of monomers, herbs and compound icariin after transport content. RESULTS: Icariin increased in the intestine during the absorption and transport process and may be excreted by P-gp transporter in Caco-2 cells. Compared with Icariin in Epimedium monomer and Epimedium extract, Meoyiyuan Tablet can significantly increase the transport of Icariin and reduce the efflux of Icariin at the same time. CONCLUSION: In brain-derived tablets, due to reduced icaritin efflux, it can be considered that other drugs in the prescription inhibit the efflux of icariin through compound compatibility, so that it can better play a pharmacological effect.