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[目的]利用近红外漫反射光谱技术建立快速简捷鉴别小麦抗旱性的评价方法。[方法]选择36个不同抗旱类型的小麦品种为试验材料,依据GB/T 21127-2007小麦抗旱性鉴定评价技术体系规范要求,以抗旱指数为指标,在2007~2009年2个生长年度,对试验材料进行旱棚抗旱性分类分级鉴定研究,并利用FOSS NIRSystems5000型近红外全光谱谷物品质分析仪,对旱棚收获后的小麦籽粒样品进行实验室光谱采集、建库和验证分析,同时进行定性分析和全光谱相关性研究。[结果]试验得到的拟合方程参数中的决定系数(RSQ)=0.697 5、交互检验决定系数(1-VR)=0.600 2,方程拟合度高,品种间近红外漫反射光谱差异显著,且与抗旱指数具有显著或极显著的相关性。[结论]利用近红外漫反射光谱技术测定评价小麦的抗旱性具有简单方便、准确快捷、全籽粒无破损等优点,是一项检测小麦抗旱性切实可行的简捷技术,对全面建立小麦抗旱性评价指标技术体系和育种材料早代鉴别具有重要的实用价值。
[Objective] The research aimed to establish a rapid and simple method for evaluating drought resistance of wheat by using near-infrared diffuse reflectance spectroscopy. [Method] Thirty-six wheat cultivars with different types of drought-resistance were selected as test materials. According to the requirements of GB / T 21127-2007 wheat drought resistance identification and evaluation technical system specification, drought resistance index was used as index in 2007-2009 growing season. The experiment materials were used to classify and classify the drought-resistance of dryhouse. The FOSS NIRSystems5000 near-infrared full spectrum grain analyzer was used to collect, build and validate the wheat grain samples after harvesting in the greenhouse. At the same time, Analysis and Full Spectrum Correlation Studies. [Result] The coefficient of determination (RSQ) was 0.697 5 and the reciprocal coefficient of determination test (1-VR) was 0.600 2. The fitting degree of the equation was high, the difference of the near-infrared diffuse reflectance spectra was significant, And had significant or extremely significant correlation with drought-resistance index. [Conclusion] Using near infrared diffuse reflectance spectroscopy to evaluate the drought resistance of wheat was a simple, convenient, accurate and quick method for determining the drought resistance of wheat. It is a simple and feasible technique to detect the drought resistance of wheat. Indicator system and early identification of breeding materials has important practical value.