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目的:用UHUPLC法与HPLC法对比测定金银花药材中绿原酸的含量。方法:分别建立了UHUPLC和HPLC测定金银花药材中绿原酸含量的方法。UHUPLC:色谱柱Agilent ZORBAX RH C18(2.1 mm×50 mm,1.8μm),流速0.2 mL/min,进样量0.5μL;HPLC:色谱柱Agilent ZORBAX XDB C18(4.6 mm×150 mm,5.0μm),流速1.0 mL/min,进样量5.0μL。流动相为乙腈(B)-0.2%磷酸水(A)(13∶87)等度洗脱,检测波长327 nm,柱温30℃。结果:2种方法所得含量测定结果相近。UHUPLC法和HPLC法加样回收率的RSD分别是0.31%和0.33%。结论:UHUPLC较HPLC法更加简便迅速,大大提高了分析的通量、灵敏度、速度,同时减少了有机溶剂的消耗。UHUPLC法可以替代HPLC法,为金银花药材质量控制提供新的检测方法和科学依据。
Objective: To compare the content of chlorogenic acid in honeysuckle with UHUPLC method and HPLC method. Methods: The method of UHPLC and HPLC determination of chlorogenic acid in honeysuckle was established. UHUPLC: column Agilent ZORBAX RH C18 (2.1 mm × 50 mm, 1.8 μm) with a flow rate of 0.2 mL / min and an injection volume of 0.5 μL; HPLC: Agilent ZORBAX XDB C18 column (4.6 mm × 150 mm, 5.0 μm) Flow rate 1.0 mL / min, injection volume 5.0 μL. The mobile phase consisted of isocratic elution of acetonitrile (B) -0.2% phosphoric acid (A) (13:87) at a detection wavelength of 327 nm at a column temperature of 30 ° C. Results: The results obtained by the two methods were similar. The RSDs of UHUPLC and HPLC recoveries were 0.31% and 0.33%, respectively. Conclusion: UHUPLC is more simple and rapid than HPLC, greatly improving the throughput, sensitivity and speed of analysis, and reducing the consumption of organic solvents. UHUPLC method can replace the HPLC method for honeysuckle herbs quality control to provide a new detection method and scientific basis.