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目的:制备白藜芦醇脂质体并对其体外抗肿瘤作用进行评价。方法:通过薄膜分散-硫酸铵梯度法制备白藜芦醇脂质体,使用粒度仪对脂质体进行表征;确定C6胶质瘤细胞对数生长期;通过磺酰罗丹明B蛋白(SRB)法评价游离白藜芦醇及其脂质体对C6胶质瘤细胞的抗增殖作用,利用流式法考察白藜芦醇及其脂质体对C6胶质瘤的凋亡作用,以及C6胶质瘤细胞对白藜芦醇和其脂质体的摄取作用。结果:白藜芦醇脂质体的平均粒径(139.97±0.64)nm,Zeta电位(-7.00±0.74)m V;游离白藜芦醇和白藜芦醇脂质体对C6胶质瘤细胞的抑制率最高分别可达(73.30±0.56)%和(91.70±0.60)%,差异有统计学意义(P<0.05);游离白藜芦醇和白藜芦醇脂质体对C6胶质瘤细胞的诱导凋亡率分别为(20.03±0.85)%和(27.18±0.96)%,差异有统计学意义(P<0.05);C6胶质瘤细胞对游离白藜芦醇和白藜芦醇脂质体的摄取率分别为(67.79±1.19)%和(77.61±1.67)%。结论:相比于游离的白藜芦醇,白藜芦醇脂质体对C6胶质瘤细胞的抗增殖作用更明显,具有更强的诱导C6胶质瘤细胞凋亡作用。白藜芦醇脂质体可以更多地被C6胶质瘤细胞摄取,这在一定程度上促进了其对C6胶质瘤细胞的抗增殖作用和诱导凋亡能力。说明白藜芦醇脂质体具有较强的体外抗C6胶质瘤作用。
OBJECTIVE: To prepare resveratrol liposomes and evaluate their anti-tumor effects in vitro. Methods: The resveratrol liposomes were prepared by thin-film dispersion-ammonium sulfate gradient method. The liposomes were characterized by particle size analyzer. The number of C6 glioma cells was determined by logarithmic growth phase. By sulforhodamine B protein (SRB) To evaluate the antiproliferative effect of resveratrol and its liposome on C6 glioma cells by flow cytometry and the apoptosis effect of resveratrol and its liposome on C6 glioma by flow cytometry, Uptake of Resveratrol and Its Liposomes by Tumor Cells. Results: The average particle size of resveratrol liposomes was (139.97 ± 0.64) nm, the Zeta potential was (-7.00 ± 0.74) mV. The free resveratrol and resveratrol liposomes had the best effect on C6 glioma cells (73.30 ± 0.56)% and (91.70 ± 0.60)% respectively, the difference was statistically significant (P <0.05). Resveratrol and resveratrol liposomes could inhibit the proliferation of C6 glioma cells The apoptotic rate was (20.03 ± 0.85)% and (27.18 ± 0.96)%, respectively, with significant difference (P <0.05). C6 glioma cells had no effect on the expression of free resveratrol and resveratrol liposome The uptake rates were (67.79 ± 1.19)% and (77.61 ± 1.67)%, respectively. CONCLUSION: Compared with free resveratrol, resveratrol liposomes have more obvious antiproliferative effect on C6 glioma cells and have stronger effect of inducing C6 glioma cell apoptosis. Resveratrol liposomes can be more uptake by C6 glioma cells, which to some extent promoted its anti-proliferative effect on C6 glioma cells and induced apoptosis. Resveratrol liposomes have a strong in vitro anti-C6 glioma effect.