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目的:观察不同应激方法制作中医郁证大鼠模型的可靠性,并了解调脂积对郁证大鼠模型血管内皮功能的影响。方法:Wistar老年大鼠共40只,随机分为正常空白组、慢性持续性温和刺激模型组、间断强刺激模型组、间断强刺激+调脂积干预组、慢性持续性温和刺激+调脂积干预组。适应性喂养后,给予除空白组外的其它组别予应激刺激(包括冰水游泳、热刺激、禁食、禁水、夹尾、昼夜颠倒、高速水平摇晃、频闪灯照射,但程度及频次不同),2个药物干预组在应激刺激的基础上喂服调脂积冲剂。之后观察各组大鼠体质量变化、糖水偏嗜等一般情况,同时检测血管活性物质(NO、ET-1)水平,电镜下观察主动脉超微结构。结果:给予不同应激刺激56 d后,2个模型组与空白组比较,糖水偏嗜度均有降低趋势,但无明显差异(P>0.05);2个药物干预组与对应模型组比较,糖水偏嗜度较模型组有明显升高,差异明显(P<0.05)。2个模型组与空白组比较,NO水平明显升高(P<0.05);2个药物干预组与对应模型组比较,NO水平有下降趋势,差异显著(P<0.05)。2个模型组与空白组比较,ET-1水平明显升高(P<0.05);2个药物干预组与对应模型组比较,ET-1水平呈下降趋势,但差异不明显(P>0.05)。同时间断强刺激模型组及间断间断强刺激+调脂积干预组大鼠血管内皮超微结构出现变化。结论:间断强刺激与慢性持续性温和刺激均可用于制作中医郁证模型,并可引起血管内皮功能的变化,可能以此途径影响心血管疾病的发病及预后;调脂积冲剂对大鼠的郁证及相关血管内皮功能及结构的有干预作用。
OBJECTIVE: To observe the reliability of different stress methods in making rat model of TCM syndrome of stagnation of qi stagnation and to understand the effect of lipid-regulating product on the function of vascular endothelium in rat model of stagnation syndrome. Methods: A total of 40 aged Wistar rats were randomly divided into normal blank control group, chronic persistent mild stimulation model group, intermittent strong stimulation model group, intermittent strong stimulation + lipid regulation intervention group, chronic persistent mild stimulation + lipid regulation product Intervention group. After adaptive feeding, other groups except the blank group were given stimulation (including ice-water swimming, thermal stimulation, fasting, water forbidden, clip tail, circadian rhythm, high speed shaking, And frequency of different), two drug intervention group on the basis of the stress stimulation fed tonicity Ji granules. The changes of body weight and glycosuria of rats in each group were observed. The levels of NO and ET-1 were measured at the same time. The ultrastructure of the aorta was observed under electron microscope. Results: Compared with the blank control group, the degree of glycemic deviations tended to decrease, but no significant difference (P> 0.05) between the two model groups and the blank control group after 56 days of different stress stimulation. Compared with the corresponding model group, The degree of glycemic index was significantly higher than that of the model group (P <0.05). Compared with the blank group, the levels of NO in the two model groups were significantly increased (P <0.05). Compared with the model group, the levels of NO in the two drug intervention groups showed a decreasing trend (P <0.05). The levels of ET-1 in the two model groups were significantly higher than those in the blank group (P <0.05). Compared with the model group, the levels of ET-1 in the two drug intervention groups showed a decreasing trend, but the difference was insignificant (P> 0.05) . At the same time, the ultrastructural changes of vascular endothelial cells were observed in the model rats of intermittent strong stimulation and intermittent strong stimulation + lipid-regulating intervention. Conclusion: Both intermittent and chronic persistent mild stimulation can be used to make TCM syndrome model and induce changes of vascular endothelial function, which may affect the incidence and prognosis of cardiovascular diseases in this way. Syndromes and related vascular endothelial function and structure of the intervention.